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7 Jan 2013

Study of Mechanism of Binahong (Anredera Cordifolia) Leaves Extract and Corn Hair (Zea Mays) Extract And Its Combination on Renal Improvement of Rat

Renal failure is a disease that until now has no cure. Renal replacement therapy, such as dialysis and transplantation, may inhibit the disease’s progression. However, they require a relatively high cost. Therefore, in the process of therapy, it is required to utilize the cheaper alternative therapies which are derived from traditional medicine that are capable to inhibit the development of renal failure. In this regard, this present study was conducted to evaluate the effect of ethanol extract of corn silk (Zea mays L.), binahong (Anredera cordifolia), and its combination against gentamicin-piroxicam induced nephrotoxicity because both corn silk and binahong have already known to have antirenal failure activity in its single use. The use of gentamicin (100 mg/kg bw; intraperitoneally) and piroxicam (3.6 mg/kg bw; orally) combination for seven days has been proved to establish a rat model of renal failure. Extract (75 mg/kg bw for corn silk and 100 mg/kg bw for binahong; orally) was given each day for four weeks since the 8th day. Evaluation of renal function was performed by measuring serum creatinine levels, renal oxidative stress, and renal histology.

Figure 1. Microscopic cross section of the renal cortex. Notes: a) negative control group, b) the positive control group, c) The corn test group d) The binahong test group e) The corn binahong half dose test group f) The corn binahong one dose test group.

Treatment with Zea mays L. and Anredera cordifolia, and its combination extract significantly restored renal function which illustrated by serum creatinine levels. It also reduced lipid peroxidation and enhanced SOD and catalase activities. Histological found similar results which showed improvement in renal cells after administration of the extract compared to the group which was not given extract. However, the application of combination extract did not give any superior effect. By using rat model, Zea mays L., Anredera cordifolia and its combination extract provided improvement against renal toxic effect of gentamicin-piroxicam.

LIST OF RESEARCH OUTPUT
1. Levina Ferdiana, Elin Yulinah Sukandar, Joseph I. Sigit, Effect of Corn Silk (Zea mays L.) on Gentamicin-Piroxicam Induced Renal Dysfunction in Rats, Seminar cum Workshop ”Women Health: Towards a Better Understanding and Enviroment for the Future Generation”, Jakarta, November 2011.
2. Calon magister/ S-2 (Levina Ferdiana)

HEAD OF RESEARCH TEAM : Prof.Dr. Elin Yulinah Sukandar
TEAM MEMBERS : Dr. Joseph I. Sigit, Msi., Levina Ferdiana Adiwibowo
OFFICIAL ADDRESS : Pharmacology- Clinical Pharmacy Research Group, School of Pharmacy ITB, Pharmaceutical Biology Research group, School of Pharmacy ITB
EMAIL : elin@fa.itb.ac.id